We’ve just released our software for offline analysis of blood vessel images. The offline analyzer can create diameter recordings from a variety of imaging data. It works with pressure myography video files, fluorsence imaging recordings (en face arteries), and even ultrasound imaging.
We are quite proud of this package, as it speeds up our analysis significantly and allowed us to easily analyze old data. If you want to measure blood vessel diameter in video recordings, download our open source (free!) software a try. Get it here.
VasoTracker Offline Analyzer 1.0.1 (27/04/2019)
Requires Microsoft Windows 10 Pro 64-bit & 8GB of RAM.
Measure blood vessel diameter from a variety of imaging data (myography, fluorescence imaging, ultrasound).
The Pachter lab at Caltech has shared its design for the Poseidon project. Poseidon is a 3D-printed syringe pump and microscope system designed for microfluidic experiments, and it is described in a preprint published on bioRxiv. The complete system is controlled by a tiny Raspberry Pi computer and comes with a 7″ touchscreen interface!
Pressure myographs require some way to image the blood vessel mounted in the chamber. We’ve been recommending cheap upright AmScope microscopes that you can get on Amazon, or Nikon’s cheapest inverted offerings. But we thought it would be nice if VasoTracker had its own open source microscope. So we’ve been adapting the Poseidon microscope design for use with our pressure myographs. We’ve even rewritten our diameter tracking software to run on the Raspberry Pi.
All in, the microscope costs less than £150 to make! Check back here soon for an update!
If you have some blood vessel imaging data, you might be in need of some analysis software. With our Offline Analyzer, you can extract diameter measurements from a wide range of blood vessel images is easy. There are options to analyze pressure myography, fluorescence, and even ultrasound data!
Have other imaging data that you want to analyze? We want our software to be as useful as possible, so get in touch and let us know!
We think VasoTracker is the first open source system designed for vascular physiology. Our description has been published at Frontiers in Physiology. Check it out here:
Penelope F. Lawton, Matthew D. Lee, Christopher D. Saunter, John M. Girkin, John G. McCarron and Calum Wilson (2019). VasoTracker, a low-cost and open source pressure myograph system for vascular physiology. Frontiers in Physiology doi: 10.3389/fphys.2019.00099