Automatic analysis of blood vessel diameter in video recordings (including ultrasound!)

Pressure myography, fluorescence, and ultrasound blood vessel diameter tracking
Pressure myography, fluorescence, and ultrasound blood vessel diameter tracking

VasoTracker Offline Analyzer 1.0.1: offline diameter tracking software

We’ve just released our software for offline analysis of blood vessel images. The offline analyzer can create diameter recordings from a variety of imaging data. It works with pressure myography video files, fluorsence imaging recordings (en face arteries), and even ultrasound imaging.

We are quite proud of this package, as it speeds up our analysis significantly and allowed us to easily analyze old data. If you want to measure blood vessel diameter in video recordings, download our open source (free!) software a try. Get it here.

VasoTracker Offline Analyzer 1.0.1 (27/04/2019)

Requires Microsoft Windows 10 Pro 64-bit & 8GB of RAM.

Features:

      • Measure blood vessel diameter from a variety of imaging data (myography, fluorescence imaging, ultrasound).
      • .tiff and .avi file compatibility
      • Many adjustable settings!

 

VasoTracker 1.0.3: live diameter tracking software update

VasoTracker 1.0.3 live diameter tracking software

We’ve updated our software. Get the latest version below!

VasoTracker 1.0.3 (26/04/2019)

Requires Microsoft Windows 10 Pro 64-bit & 8GB of RAM.  μManager and camera drivers may have other requirements.

MAKE SURE YOU INSTALL THE PREREQUISITES (SEE HERE)

Compatible cameras:

      • Any camera supported by µManager.

Updates:

      • Ability to alter inner diameter detection mode
      • Alter smoothing
      • Camera set by µManager .cfg file
      • Fixed some issues installing on certain 64-bit systems.

 

Pressure myography on an open source, 3D-printed microscope using Raspbery PI

 

The Pachter lab has shared their designs for the Poseidon project created at the California Institute of Technology. Poseidon is a 3D-printed  syringe pump and microscope system designed for microfluidic experiments, and it is described in a preprint published on bioRxiv. The complete system is controlled by a tiny Raspberry Pi computer and comes with a 7" touchscreen interface!We've been adapting the Poseidon microscope design for use with our pressure myographs. We've even rewritten our diameter tracking software to run with on the Raspberry Pi. 

The Pachter lab at Caltech has shared its design for the Poseidon project. Poseidon is a 3D-printed  syringe pump and microscope system designed for microfluidic experiments, and it is described in a preprint published on bioRxiv. The complete system is controlled by a tiny Raspberry Pi computer and comes with a 7″ touchscreen interface!

Pressure myographs require some way to image the blood vessel mounted in the chamber. We’ve been recommending cheap upright AmScope microscopes that you can get on Amazon, or Nikon’s cheapest inverted offerings. But we thought it would be nice if VasoTracker had its own open source microscope. So we’ve been adapting the Poseidon microscope design for use with our pressure myographs. We’ve even rewritten our diameter tracking software to run on the Raspberry Pi.

All in, the microscope costs less than £150 to make! Check back here soon for an update!

COMING SOON! Analyze blood vessel images with our open source analyzer

If you have some blood vessel imaging data, you might be in need of some analysis software. With our Offline Analyzer, you can extract diameter measurements from a wide range of blood vessel images is easy. There are options to analyze pressure myography, fluorescence, and even ultrasound data!

Have other imaging data that you want to analyze? We want our software to be as useful as possible, so get in touch and let us know!

 

 

 

 

VasoTracker is alive

We think VasoTracker is the first open source system designed for vascular physiology. Our description has been published at Frontiers in Physiology. Check it out here:

  • Penelope F. Lawton, Matthew D. Lee, Christopher D. Saunter, John M. Girkin,  John G. McCarron and Calum Wilson (2019). VasoTracker, a low-cost and open source pressure myograph system for vascular physiology. Frontiers in Physiology doi: 10.3389/fphys.2019.00099